Preventive effect of Fisetin on Cardiac markers, Lipid peroxides and Antioxidants in normal and Ischemia-Reperfusion induced Myocardial infarction in rats

Abstract

Author(s): Uday Raj Sharma*, EgaSowparnika, Surendra V., Nageena Taj, Manjunath P.

Cardioprotective effects of Fisetin may be attributed to its antioxidant, antinflammatory andantiapoptotic activities. Wistar albino rats were randomly cleaved into 5 groups (n=10 in each group):Group I (Sham group), Group II (Control group), Group III (Vehicle treated), Group IV (Fisetin 10 mg/kg)and Group V (Fisetin 20 mg/kg), left coronary artery occluded for 30 min, followed by 4 hours ofanesthetized rat reperfusion. The concentration of myocardial enzymes (LDH, CKMB), antioxidants (SOD,CAT, GSH, GPx, GST) and MDA levels were measured after the model was developed. Results showed thatthe rates of infarction, serum cardiac markers (CKMB and LDH), antioxidant tissue enzymes (SOD, CAT,GPx, GSH, and GST) have increased, the levels of lipid peroxides have decreased and the ultrastructuralchanges in the myocardil organization have improved significantly in Group IV and V, when compared with the Group I.Fisetin’s cardioprotective effect in this model was slightly changed via reduced infarctvolume, decreased CKMB and LDH release increased the capacity of antioxdants (SOD, CAT, GPx, GSH,and GST), decreased lipid peroxidation (MDA) and further confirmed by histopathological reports.