A Validated RP HPLC Method for Simultaneous Estimation of Nebivolol and Hydrochlorothiazide in Combined Dosage Forms

Abstract

Author(s): Swethanagini V, Vasanth PM, Ramesh T, Ramesh Malothu

An accurate, Precise, Simple and Economical High Performance Liquid Chromatographic method for the estimation of Nebivolol and Hydrochlorothiazide was developed and validated. The determination was performed by the using of two phases one is stationary phase it’s a Thermo BDS Hypersil C18 column having 250 x 4.6mm 5μ, andanother one is mobile phase containing 0.1N phosphate buffer and Acetonitrile at the ratio 50:50%v/v. The flow rate was 1ml/min and effluents were monitored at 282nm. The retention time of Nebivolol and Hydrochlorothiazide was 3.5 and 5.4 min respectively. The developed method was validated for specificity, system suitability, precision, linearity, accuracy, Limit of Detection, Limit of Quantification, robustness, and ruggedness. Recovery of Nebivolol and Hydrochlorothiazide in formulations was found to be in the range of 100%, 100%, and 101%respectively. And the correlation coefficient was 0.999. Hence, it was concluded that the developed method is suitable for routine analysis of these combination due to its less analysis time